Laboratoř RT-QuIC, First Faculty of Medicine, Charles University Prague
Research objectives:
– Increase our understanding of the fidelity of amyloid seed induced conformational change and aggregation of NDPP specific proteins. Evaluate if the conformational signature of pathological seed can be traced in the structure of aggregated product of RT-QuIC reaction and utilized to improve diagnostics of NDPP. Study the effect of the aggregation conditions on structure of resulting amyloid fibrils.
– Develop and validate variants of RT-QuIC assay for synucleinopathies, tauopathies and prion diseases utilizing clinically relevant patient samples. In collaboration carry out retrospective and prospective studies to confirm their diagnostic potential. Contribute to the implementation of RT-QuIC assay into routine clinical laboratory diagnostic practice.